Table 3. Plasmid constructions used in two-hybrid assays.
| Plasmid | Construction | Complementation of (mutant)* |
|---|---|---|
| pMTX502 | pT25::trwBΔN75 | Not tested |
| pMTX503 | pT18::trwBΔN75 | Not tested |
| pMTX504 | pT25::trwC | <10−4 (pSU1445) |
| pMTX505 | pT18::trwC | <10−4 (pSU1445) |
| pMTX506 | pT25::trwA | Not tested |
| pMTX507 | pUT18::trwA | 0.01 (pSU1092) |
| pMTX508 | pUT18C::trwA | 0.003 (pSU1092) |
| pMTX512 | pUT18::trwB | 2.0 (pSU1443) |
| pMTX513 | pUT18C::trwB | 5.5 (pSU1443) |
| pMTX514 | pT25::trwB | 1.8 (pSU1443) |
| pMTX583 | pUT18::trwK | 0.003 (pSU4133) |
| pMTX584 | pT25::trwK | <10−4 (pSU4133) |
| pMTX585 | pUT18C::trwK | 0.005 (pSU4133) |
| pMTX631 | pUT18C::trwE | 0.7 (pSU4134) |
| pMTX632 | pT25::trwE | 0.8 (pSU4134) |
| pMTX634 | pT25::trwD | 0.005 (pSU4132) |
| pMTX635 | pT18::trwD | 0.01 (pSU4132) |
| pMTX643 | pUT18C::trwEΔN64 | Not tested |
| pMTX644 | pUT18C::traJ | Not tested |
| pMTX651 | pT25::traJ | Not tested |
| pMTX667 | pUT18C::traF | Not tested |
| pMTX668 | pT25::trwEN174 | Not tested |
| pMTX669 | pUT18C::trwEN174 | Not tested |
| pMTX674 | pT25::traF | Not tested |
| pMTX677 | pUT18C::pilX10 | Not tested |
| pMTX679 | pUT18C::taxB | Not tested |
| pMTX680 | pT25::taxB | Not tested |
*
Plasmids in boldface were introduced with the plasmid indicated in parentheses in strain D1210. These cells as donors were mated with strain DH5α. Figures show the percentage of transconjugants as compared to the transfer level of pSU2007 (100%). Plasmids in parentheses are all transfer-deficient except for pSU1092, which has a residual 1% conjugation frequency.