Table 4. Conjugation assays.

		TRA*			MOB†			RLX‡
Helper	Mobilizable	W	N	X	W	N	W	N	Transfer
pSU2007	pSU1423	+			+				5,2 × 10−1
pSU2007	pSU4280	+				+			1,8 × 10−2
pSU2007	pSU1404	+					+		6,1 × 10−1
pSU2007	pMTX681	+						+	<9 × 10−8
pKM101	pSU1423		+		+				1,3 × 10−1
pKM101	pSU4280		+			+			2,8 × 10−1
pKM101	pSU1404		+				+		<6 × 10−7
pKM101	pMTX681		+					+	1,0 × 10−1
R6K-drd	pSU1423			+	+				1,6 × 10−3
R6K-drd	pSU4280			+		+			3,4 × 10−5

Donors were derivatives of strain DH5α harboring the indicated helper and mobilizable plasmids (first two columns). The recipient was strain UB 1637. Transfer frequencies are indicated as the number of transconjugants per donor. The table indicates the parts of the conjugal machinery that are present in each tested plasmid pair. W, N, and X refer to the specific mating system of the parental conjugal plasmid.

^*TRA = complete transfer system = MOB + T4SS.

^†MOB = RLX + CP; MOB_W = oriT_W + trwA + trwB + trwC; MOB_N = oriT_N + traK + traJ + traI.

^‡RLX = oriT + relaxase + accessory nicking protein; RLX_W = oriT_W + trwA + trwC; RLX_N = oriT_N + traK + traI.