Fig. 3.

Thrombin digestion of a modified variant of DsbD. FED513 cells containing the plasmid pFK277 were grown as indicated before and alkylated with N-ethylmaleimide as specified in Methods. Samples were digested or not by thrombin protease and resuspended in SDS/sample buffer. When indicated, aliquots were reduced with 50 mM DTT. Proteins were visualized by Western blotting using antibodies against the α (A) and γ (B) peptides.