Table 1. Hill coefficients for the Ca2+ dependence of CaMKII autophosphorylation at varying concentrations of CaMKII and PP1.
[CaMKII], μM
|
|||
---|---|---|---|
[PP1], μM | 0.2 | 1 | 5 |
0 | 4.4 ± 0.6 | 4.9 ± 0.3 | 5.4 ± 0.4 |
0.5 | 7.7 ± 0.8 | 6.6 ± 1.7 | 8.9 ± 1.8 |
1.25 | 6.5 ± 0.5 | 8.6 ± 2.5 | 6.9 ± 1.6 |
2.5 | — | 5.9 ± 0.3 | 8.9 ± 2.2 |
Each nH value represents the mean obtained from at least two Ca2+ titrations. nH values were often obtained at several time points, and these values were averaged to obtain the final value. Uncertainties are the SD.