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. 1998 Mar 3;95(5):2417–2422. doi: 10.1073/pnas.95.5.2417

Figure 2.

Figure 2

Northern hybridization analysis of cys-3+ mRNA levels in the scon-2 mutant strain D143I. Poly(A)+ mRNA was isolated from N. crassa wild-type, D143I, Δscon-2, and Δcys-3(18–4) strains grown under low and high sulfur conditions (L has been used to designate low sulfur growth conditions; H designates high sulfur growth conditions). Northern blots were prepared and probed with 32P-labeled cys-3+ and am+ DNA. The am+ gene (10) served as a control to ensure comparability between samples. Lanes 1–2, mRNA from wild-type N. crassa; lanes 3–4, mRNA from the site-directed F-box mutant strain D143I; lanes 5–6, mRNA from the scon-2 deletion strain Δscon-2; lanes 7–8, mRNA from the cys-3 deletion strain Δcys-3(18–4). As indicated in lanes 3–4, cys-3+ gene expression could not be detected in the D143I mutant strain under either low or high sulfur growth conditions.