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. 1998 Mar 3;95(5):2423–2428. doi: 10.1073/pnas.95.5.2423

Figure 1.

Figure 1

Competitive RT-PCR assay of constitutive hsr-omega RNA abundance in thermoresistant lines. (a) RT-PCR strategy. Positions of forward (F) and reverse (R) primers to uniquely detect each transcript (omega-c = ωcF, ωcR; omega-n = ωnF, ωnR) are indicated adjacent to the transcripts. These underlie the depicted genomic structure of hsr-omega. ORF, potential ORF (10). (b) Gel showing RT-PCR amplification of omega-c over a range of competitor concentrations that allowed estimation of absolute transcript levels (track M, 100-bp ladder size standard; RT-, control lacking RT, see Materials and Methods). (c) Equivalent gel showing RT-PCR amplification of omega-n.