Table 1.

Partial purification of sucrose-phosphate synthase from Anabaena 7119

Steps	Total protein, mg	Total activity, nkat*	Recovery, %	Specific activity, nkat/mg	Purification, -fold
Crude extract	92.0	2.80	100	0.03	1
DEAE–Sephacel (SPS-I)	6.5	2.10	75	0.32	10
Sephadex G-100 (SPS-I)	0.9	0.58	20  (27)	0.64	21
DEAE–Sephacel (SPS-II)	1.83	0.74	26	0.40	13
Sephadex G-100 (SPS-II)	0.12	0.50	17  (67)	4.17	139

Percentages in parentheses indicate recovery from previous step. 

^*Amount of enzyme that converts 1 nmol of substrate to product in 1 sec.