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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 22;1769(4):209–219. doi: 10.1016/j.bbaexp.2007.02.002

Fig. 6.

Fig. 6

FLJ11259 contains a functional p53 responsive element. (A) Reporter assay of HCT116 p53+/+ and HCT116 p53−/− cells transfected with either control pGL3-promoter reporter (pGL3-luc) or FLJ11259 reporter (FLJ-luc: pGL3-promoter containing a 600 bp region of FLJ11259 surrounding the p53 response element). (B) Reporter activity of HCT116 p53+/+ cells transfected with the pGL3-luc or FLJ-luc reporter and either a control vector or dominant negative p53 (DNp53). FLJ-luc activity is diminished in the presence of DNp53. (C) FLJ-luc reporter activity in NT2/D1 cells. (D) Loss of FLJ-Luc reporter activity in HCT116 p53+/+ cells when the p53 RE is mutated. The core C and G in each half site of the p53RE was mutated in the context of the 600-bp upstream element as described in Methods. (E) HCT116 p53+/+ and HCT116 p53−/− cells were transfected with FLJp53RE or the corresponding empty tk-luc vector and DNp53 or p53 expression constructs. Activity of the FLJp53RE reporter occurs in a p53-dependent manner in both cell systems. Data is representative of three independent experiments each performed in triplicate, error bars are standard deviation.