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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 22;1769(4):209–219. doi: 10.1016/j.bbaexp.2007.02.002

Fig. 7.

Fig. 7

Overexpression of FLJ11259 is associated with increased colony formation. (A) FLJ11259 overexpression increases colony formation after G418 selection of Saos2 cells transfected with FLJ expression vectors (FLJ-1 and FLJ-2 are two independent plasmid preparations of pcDNA3.1-FLJ11259) compared to empty vector (pcDNA3.1). Cells were stained with Giemsa to visualize colonies. (B) Number of colonies formed in Saos2 cells transfected with empty vectors (pcDNA3.1 or pCSN3) or FLJ expression vectors (FLJ-1 and FLJ-2) or p53. Triplicate plates were manually counted for each data point. Error bars are standard error of the mean. Statistical analysis performed with Student-Newman-Keuls multiple comparisons test, p<0.001 compared to pcDNA3.1 vector control.