Table 1.
Number of Colonies per T75 flask | ||||||
---|---|---|---|---|---|---|
Cell Line | Tx # | pcDNA3.1 | FLJ-1 | FLJ-2 | pCSN3 | p53 |
Cos-7 | 1 | 7 | 297 | 256 | ND | ND |
2 | 34 | 463 | 565 | ND | ND | |
NT2/D1 | 1 | 19 | 74 | 138 | ND | ND |
2 | 31 | 78 | 127 | ND | ND | |
3 | 26 | 77 | 102 | ND | ND | |
Saos2 | 1 | 649 | 1645 | 1434 | 374 | 11 |
2 | 602 | 1461 | 1415 | 496 | 8 | |
3 | 467 | 1750 | 1145 | 466 | 11 |
Cell lines were transfected with FLJ or p53 expression plasmids or corresponding empty vectors (pcDNA3.1 and pCSN3). Identical cell numbers were plated into G418 media. Colonies were counted approximately 2 weeks later. ND – Not determined. FLJ-1 and FLJ-2 are two independent plasmid preparations of pcDNA3.1-FLJ11259. Tx is transfection number.