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. 2003 Sep 15;112(6):924–934. doi: 10.1172/JCI15543

Figure 6.

Figure 6

Effects of BMP and ca-ALK-3 overexpression on osteoblast differentiation. (a) Concentration-dependent stimulation of ALP activity in mature OB-6 osteoblasts by BMP-2 and BMP-4, expressed as absolute values. (b) Concentration-dependent inhibition of ALP activity by noggin or PEG-noggin in the presence of a fixed dose of BMP-2 or BMP-4 (100 ng/ml), expressed as a percentage of the control value. (c) Anti-HA–immunostained U-33 cells documenting constitutively active BMP type 1A receptor (ca-ALK-3) expression; the left panel (Vector) is a negative control. (d) Effect of increasing concentrations (0–100 ng/ml for 3 days) of PEG-noggin in vector or ca-ALK-3–overexpressing U-33 cells as assessed by ALP activity (given as mean ± SEM), expressed as absolute values. *P < 0.05, significantly different from untreated control (Vector) cells; #P < 0.05, significantly different from ca-ALK-3 at 0 concentration, by ANOVA.