Figure 8.

Phenotype of an 8-month-old noggin-overexpressing mouse. (a) Radiology of the femur; (b) bone mineral density of the femur, tibia, spine, and total body; and (c) H&E staining of femur were performed to show differences in WT and noggin transgenic mice. (d) Comparison of bone marrow cell cultures isolated from WT and noggin TG mice to evaluate ALP-positive CFU-F, CFU-OB, and (e) TRAP-positive osteoclast formation. (f) The percentage of doubly-labeled bone surface to total bone surface and (g) the rate of bone formation as determined by the distance between calcein labeling lines in long bone (femur and tibia), spine, and calvaria from 8-month-old WT or noggin-overexpressing TG mice. Calvarial sections stained with H&E were analyzed to determine marrow space as a percentage of total bone area (h). Results are expressed as mean ± SEM. Statistics by ANOVA between WT and noggin TG mice. *P < 0.05.