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. 2003 Sep 15;112(6):843–852. doi: 10.1172/JCI17647

Figure 2.

Figure 2

JunD expression increases after nephron reduction. (a) Immunoblot analysis of JunD expression in control nonoperated (designated 0) and remnant kidneys of JunD+/+ mice. Protein expression was analyzed before surgery (day 0) and 7, 14, 30, and 60 days after nephron reduction. Extracts of JunD–/– kidneys served as negative controls (C–). (b) Immunohistochemical analysis of JunD–β-gal reporter gene expression in control nonoperated (designated 0) and remnant kidneys of JunD–/– mice. β-gal staining was performed before surgery (day 0) and 7, 14, 30, and 60 days after nephron reduction. The top panels show staining in control and remnant kidneys at day 60 (× 200). The bottom panel shows the number of β-gal–positive cells per tubular section at each experimental time point. Data are mean ± SEM; n = 4–6 for each point. ANOVA, followed by Tukey-Kramer test: **P < 0.005, ***P < 0.001, remnant versus control kidneys.