Figure 2.

Specific recognition of 2.5mi⁺ T cells by A^g7/2.5mi multimers. (a) BDC-2.5 T cell hybridoma and 19.2B T cell hybridoma (specific for glutamic acid decarboxylase peptide 282–292) were labeled with either A^g7/2.5mi tetramers (solid line) or A^g7/GPI tetramers (dotted line). (b) Staining of T cell clones specific for β cell granule membranes with A^g7/2.5mi tetramers. After 4 days of stimulation in vitro with islet-membrane preparations, BDC clones were stained with A^g7/2.5mi tetramers. (c) Inguinal LN cells from young adult BDC-2.5/NOD, KRN, and NOD (Non-Tg) mice were stained for CD4 and A^g7/2.5mi tetramers.