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Histological detection of 2.5mi+CD4+ T cells. In situ staining of 2.5mi+CD4+ T cells in PLNs (a and b) and pancreatic islets (c–e). I-Ag7/2.5mi MHC tetramer stains are shown in green (a and c). Staining with an anti-CD4 antibody is shown in red and overlaid by the tetramer staining (b and d). Insulin staining is shown in e.
