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UV–visible absorption spectra of lysyl endopeptidase digest of GFP (A) and compound 1 (B). Traces: a, GFP digest in 0.1 M NaOH; b, GFP digest in 0.1 M HCl; c, compound 1 in 2-propanol containing 0.1 M NaOH aqueous solution, 2.5%, (vol/vol); d, compound 1 in 2-propanol containing 0.1 M HCl aqueous solution, 2.5% (vol/vol). Estimated original concentration of GFP = 4.8 × 10−6 M; concentration of compound 1 = 5.0 × 10−5 M.
