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. 2003 Sep;23(18):6494–6506. doi: 10.1128/MCB.23.18.6494-6506.2003

FIG.4.

FIG.4.

RILP promotes dynamitin recruitment and displacement of phagosomes towards the MTOC. RAW cells were transfected with RILP-EGFP (A, C, and F) or RILP-C33-EGFP (B, D, and G) and allowed to internalize 3.1-μm-diameter (A, B, F, and G) or 0.8-μm-diameter (C and D) IgG-coated beads. After 50 min, the cells were fixed, permeabilized, and immunostained for either α-tubulin (A and B), γ-tubulin (C and D), or p50-dynamitin (F and G). Main panels in A and B show α-tubulin staining overlaid on DIC images. Insets show distribution of RILP-EGFP (A) or RILP-C33-EGFP (B). Bars, 10 μm. Panels C and D show smaller beads (blue) overlaid with γ-tubulin (red) and RILP (C) (green) at 30 min and RILP-C33 (D) (green) at 50 min. Asterisks indicate the location of the MTOC. Bars, 5 μm. Quantitation of bead distance from MTOC is shown in panel E. Data are means from four separate experiments. Main panels in F and G show p50-dynamitin staining. Insets show distribution of RILP-EGFP (F) or RILP-C33-EGFP (G) overlaid on DIC images. Arrowheads point to phagosomes. Bars, 10 μm.