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. 2003 Sep;23(18):6702–6712. doi: 10.1128/MCB.23.18.6702-6712.2003

FIG. 6.

FIG. 6.

IMPDH and HPRT enzymatic activities in mutant mice. (A) IMPDH enzyme activity was measured in tissues of single- and double-knockout mice. Total-cell lysates of 20 μg (thymus), 50 μg (liver, brain, and testis), and 60 μg (lung) were used for each assay. Activities from mutant mice (IMPDH type I−/− [n = 4], type I−/− type II+/− [n = 5], and type I−/− HPRT−/0 [n = 5]) were plotted as percentages of activities obtained from wild-type tissues (n = 14). (B) IMPDH enzyme activities were measured on 10 μg of splenocyte lysates from mice with an IMPDH type I−/− or type II+/− genotype before and after ConA activation and plotted as percentages of wild-type activities. (C) HPRT activities were measured from type I−/− type II+/− double-knockout mice (n = 4) and plotted as percentages of wild-type activity. Values are means ± standard deviations, with each determination performed in duplicate.