FIG. 8.

(A and B) Levels of IL-2 and IL-4 secreted by splenocytes of wild-type and type I^−/− HPRT ^−/0 mice. Splenocytes from two wild-type (wt-1 and wt-2) and two IMPDH type I^−/− HPRT^−/0 (Mut-1 and Mut-2) animals were stimulated with anti-CD3 plus anti-CD28 antibodies. Culture supernatants were collected at the indicated times and measured for the presence of IL-2 (A) and IL-4 (B). Enzyme-linked immunosorbent assays were performed in duplicate. (C and D) Splenocytes from wild-type (wt) mice were activated by antibodies in the absence (solid circles) or presence of 0.2 μM MPA (M0.2) (open circles) or 0.5 μM MPA (M0.5) (solid inverted triangles), and levels of IL-2 (C) and IL-4 (D) secretion were determined in duplicate.