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Ssk2p-independent degradation of Ssk1p. Tagged Ssk1p was expressed from pNS462 in the YPD1+ ssk1Δ hog1Δ SSK2+ (NS318), ypd1Δ ssk1Δ hog1Δ SSK2+ (NS320), YPD1+ ssk1Δ hog1Δ ssk2Δ (NS354), and ypd1Δ ssk1Δ hog1Δ ssk2Δ (NS355) strains, and the Ssk1p content was monitored. +, present; Δ, deleted.
