TABLE 2.
Plasmids used in this study
| Plasmid | Descriptiona | Source or reference |
|---|---|---|
| p416GAL1 | PGAL1URA3 CEN | 41 |
| p424GAL1 | PGAL1TRP1 2μm | 41 |
| p426TEF | PTEFURA3 2μm | 42 |
| p426GPD | PGPDURA3 2μm | 42 |
| pRS414 | TRP1 CEN | 58 |
| pRS416 | URA3 CEN | 58 |
| pRS424 | TRP1 2μm | 11 |
| pFA6a-3HA-6His-His3MX6 | 3HA-6His-TADHHis3MX6 | T. Maeda, unpublished |
| pFA6a-TRP1 | TRP1 | 34 |
| pRH1186 | pBluescript KSII ubc7::LEU2 | 17 |
| pJUN180 | pBluescript KS(−) rpn9::LEU2 | 61 |
| pRS315-SKN7 | SKN7 LEU2 CEN | 8 |
| pNS396 | pBluescript KS(+) skn7::TRP1 | This study |
| pNS252 | p424GAL1 SSK1-3HA-6His | This study |
| pNS114 | pRS414 PGAL1-SSK1-3HA-6His | This study |
| pNS476 | pRS416 YPD1 | This study |
| pFP57 | pRS416 ypd1 (H64Q) | 50 |
| pcDNA3.1/N-3×FLAG | 3×FLAG | T. Maeda, unpublished |
| pTB422 | pcDNA3.1 3×FLAG-SSK1 | This study |
| pTB426 | p416GAL1 3×FLAG-SSK1 | This study |
| pNS462 | pRS416 PGAL1-SSK1-3HA-6His | This study |
| pcDNA3-FLAG | FLAG | Gift from M. Kawabata |
| pNS454 | p426TEF SSK1-FLAG | This study |
| pNS456 | p426GPD SSK1ΔUBL-FLAG | This study |
| pNS473 | pRS416 PGAL1-SSK1 | This study |
| pHG11 | pRS424 HOG1 | 38 |
a
PGAL1, PTEF, and PGPD are, respectively, GAL1, TEF, and GPD promoters. TADH is an alcohol dehydrogenase terminator.