FIG. 4.

Real-time gene conversion assay. (A) DNA from MK203 and MK203rad24 cells was extracted at intervals after HO induction. Equal amounts of PCR products flanking the DSB were digested with BamHI. Only fragments originating from a template repaired by gene conversion can be digested. (B) Quantitation of PCR. Percent gene conversion represents the portion of PCR fragments cut by BamHI. (C and D) Exponentially growing MK203 and MK203rad24 cells were arrested at G₂/M with nocodazole (noc.) and transferred to galactose in the presence of nocodazole. Southern blot (C) and PCR (D) analyses were carried out as described above.