FIG. 4.

ssu72-G33A does not affect recognition of the CYC1 cleavage/polyadenylation site. (A) Schematic illustration of the CYC1 gene and 3′-flanking region showing the 83-bp CYCpAmin fragment inserted into the ACT-CUP reporter gene intron. Also shown are the CYCpAmax and CYCds fragments used in the TRO experiments for which results are shown in Fig. 5. (B) Copper sensitivity assay with wild type, nrd1-5, and ssu72-G33A strains harboring ACT-CUP reporter genes with the CYC1pAmin cleavage/polyadenylation element inserted into the intron in the forward or reverse orientation.