Skip to main content
. 2003 Sep;23(18):6350–6362. doi: 10.1128/MCB.23.18.6350-6362.2003

FIG. 2.

FIG. 2.

pSer112 and pSer136 cooperate in 14-3-3 binding to block dephosphorylation of BAD. (A) Lysates from NIH 3T3 cells expressing the BAD 112A mutant were incubated in phosphatase assay buffer in the absence or presence of R18 peptide and immunoblotted with anti-pSer136, anti-pSer155, and anti-BAD antibodies. (B) Lysates from NIH 3T3 cells expressing the BAD 136A mutant were subjected to in vitro dephosphorylation as in A and immunoblotted with anti-pSer112 and anti-BAD antibodies. Phosphorylation of Ser155 was barely detectable and is not shown. (C) Lysates from NIH 3T3 cells expressing the BAD 155A mutant were similarly subjected to in vitro dephosphorylation and assayed for pSer112 and pSer136 as above.