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. 2003 Sep;23(18):6553–6563. doi: 10.1128/MCB.23.18.6553-6563.2003

FIG.1.

FIG.1.

Amino acid sequence analysis of S. pombe Nbs1. Alignment was performed as described previously (64). (A) Overall structural comparison among several Nbs1 and related proteins. (B) Forkhead-associated (FHA) domain. (C) BRCA1 C-terminal (BRCT) domain. The five conserved regions, designated A to E (7), of the BRCT domain based on the hydrophobic cluster analysis (HCA) are shown by a dotted arrow. The secondary structure of the XRCC1 BRCT domain (69) is shown at the bottom of the alignment. (D) C-terminal conserved domain (CCD). Protein sequences were obtained from humans (h), mice (m), chickens (ch), Aspergillus nidulans (an), Drosophila melanogaster (dm), Danio rerio (dr), Xenopus laevis (xl), S. pombe (sp), and S. cerevisiae (sc). Color of conserved positions (at most, two deviating amino acids for the forkhead-associated domain, four deviating amino acids for the BRCT domain, and three deviating amino acids for the CCD): green, hydrophobic (FYWILMVTAC); red, acidic (DEQN); blue, basic (HKR); magenta, glycine (G); brown, serine or threonine (ST); yellow, proline (P). Sequences are denoted by species identification prefixes for Caenorhabditis elegans (ce) and Arabidopsis thaliana (at) and by protein designations for hNBS1 (GenBank BAA28616); spNBS1 (GenBank AB099299); drNBS1-N (GenBank BI984731); drNBS1-N (GenBank BM775439); xlNBS1-N (GenBank CA988284); xlNBS1-C (GenBank BG022948); scXRS2 (GenBank AAB64805); mNBS1 (GenBank AB016988); chNBS1 (GenBank AAG47947); dmNBS1, also named CG6754-PB protein (GenBank NM_143716); predicted translation products of the cosmids F37D6.1 (GenBank CAA99847) and T10M13.12 (GenBank T01512); the CAG trinucleotide repeat containing cDNA CAGF28 (GenBank AAB91434); the BRCA-associated RING finger domain protein BBARD (GenBank Q99728); the DNA repair proteins XRCC1 (GenBank A36353) and REV1 (GenBank S67255); the oncoprotein ECT2 (GenBank S32372); the breast cancer susceptibility type 1 protein BRCA1 (GenBank U14680); the radiation-sensitive checkpoint protein Rad9 (GenBank M26049); and campothecin resistance-conferring protein SCAA (GenBank AAF81094), which has moderate structural similarity to hNBS1. Numbers in the alignment denote the number of amino acids omitted.