TABLE 1.
Oligonucleotides used for PCR in this study
| Subclone | Forward oligonucleotide(s) (source), sequence(s) | Reverse oligonucleotide(s) (source), sequence(s) |
|---|---|---|
| Promoter | ||
| S | P107, CCAGATCTGCGCCAGCAGAGCCAGC | GL primer 2 (Promega), CTTTATGTTTTTGGCGTCTTCC |
| T | P110, GGGGAGCTCGCTCGGTCCGCTCGC | GL primer 2 |
| U | P111, TCCGAGCTCCGCGCCGGAGAGAGCTGC | GL primer 2 |
| V | P105, AGAGATCTGAGACGCAGCCAGCCAG | GL primer 2 |
| W | P109, CCAGGAGCTCGGCGCCACGCCGCCGAGC | GL primer 2 |
| X | RV primer 3 (Promega), CTAGCAAAATAGGCTGTCCC | P206, CGAGATCTCGCACTCAGGGACTCCGG |
| Y | RV primer 3 | P207, GTGGCCTCGAGCTCGGCGGCGTGGCGCC |
| Z | RV primer 3 | P208, GCGTGCTCGAGGCTGGCTGGCTGGCTGC |
| AA | P110 | P206 |
| BRE-1 m1 | P520, TCGAGATCTGAGACGCTGCCTGCCTGCCTGCCGGCGCCACG | GL primer 2 |
| BRE-1 m2 | P518, TCGAGATCTGAGACGCAGCCAGCCAGCCAGCTAACGCCACGC | GL primer 2 |
| BRE-1 m3 | RV primer 3 | P608, CAGATCTCGAGTCCTTAGTAATACGCTGGGGGCTCG |
| Intron | ||
| Q | GAGCTCGGAGGGGGAGACTCTGAATT | GL primer 2 on clone P |
| I-BRE (R) | CTGTTTGTCTTAGCTGTGGG | GL primer 2 on clone P |
| I-BRE M1 | I502, GGTGATTGCCTTAGCGTTTAGCTGTC; RV primer 3 | GL primer 2; I602, GACAGCTAAACGCTAAGGCAATCACC |
| I-BRE M2 | I503, GCTGTCAGACGGGCAAAAGAGGCC; RV primer 3 | GL primer 2; I603, GGCCTCTTTTGCCCGTCTGACAGC |
| I-BRE M3 | I512, GCTGTCAAGTAAACAAAAGAGGCC; RV primer 3 | GL primer 2; I612, GGCCTCTTTTGTTTACTTGACAGC |
| I-BRE M4 | I511 TGTCAGATAACACAAAGAGGCCGCC; RV primer 3 | GL primer 2; I611, CGGCCTCTTTGTGTTATCTGACAG |
| GATA-5 | ||
| Flag-GATA-5 | G5-101, GCGGTACCATGGACTACAAGGACGACGATGACAAGGGATCCTACCAA AGCTTGGCGTTAGC | G5-201, AACTCCTCCAAGAAGTCAGG |
| 1-312 | G5-101 | G5-202, GCCCTCGAGTCATCCTGAGGAGCCCTTGATTTTG |
| 1-249 | G5-101 | G5-204, GCCCTCGAGTCAGCATAGGCCGGATCTTCGG |
| 1-195 | G5-101 | G5-205, GCCCTCGAGTCACTCCCGGCCCTCACCAGG |
| Δ49-91 | G5-120, ATGCTGCCGTACCTGCCTTCGACCTTCCCGTTCGCTCACAG; G5-101 | G5-202; G5-212, CGAAGGCAGGTACGGCAGCATG |
| Δ92-139 | T7; G5-106, GCCGGATCCACAACCTACCCAGCATACATG | G5-606, CATGTATGCTGGGTAGGTTGTGGTGGCTCCCGGTGGGTG; G5-202 |
| Δ140-195 | G5-505, TGCGTCAACTGTGGAGCC; T7 | G5-202; G5-605, GGCTCCACAGTTGACGCAGGGGTACGAGGCGCCCATG |
| Δ196-220 | G5-503, CCTGGTGAGGGCCGGGAGGGCCTCTATCACAAGATG; T7 | G5-202; G5-205 |
| Δ196-249 | G5-121, CCTGGTGAGGGCCGGGAGTGCTCCAACTGCCATACTGCCAC; G5-101 | G5-202; G5-205 |
| Δ196-274 | G5-504, CCTGGTGAGGGCCGGGAGGGCCTCTACATGAAGCTC; T7 | G5-202; G5-205 |
| C253G | G5-501, TGCTCCAACGGCCATACTGC; G5-101 | G5-202; G5-601, AGTATGGCCGTTGGAGCAG |
| GATA-5/GATA-2 zinc finger chimeras | G5-101; G5-107, GCCGGGAGTGTGTCAACTGTG; G5-108, GTCTGCAACGCCT GTGGCCTCTAC | G5-209, GGCCCCACAGTTGACACACTC; G5-208, GTAGAGGCCACAGGCGTTGC; G5-202 |
| GATA-5 1-91/GATA-2 170-480 | G5-101; G2-102, GCACACCCACCGGGAGCCACCCTTTTCGGCTTCCCACCCACG | G5-213, GGTGGCTCCCGGTGGGTGTGC; G2-202, GGGCAGCCGCCGGCACATAG |
| GATA-5 1-48/GATA-2 170-480 | G5-101; G2-103, ATGCTGCCGTACCTGCCTTCGCTTTTCGGCTTCCCACCCACG | G5-212; G2-202 |