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. 2003 Sep;23(18):6672–6684. doi: 10.1128/MCB.23.18.6672-6684.2003

FIG. 4.

FIG. 4.

Subcellular localization of yNap1 derivatives in yeast cells. (A) Exponentially growing cells expressing WT Flag-yNap1, Flag-yNap1ΔNES, or Flag-yNap1 (L99A L102A) were fixed, and DNA and Flag-tagged proteins were stained with DAPI and anti-Flag antibody, respectively. (B) Amounts of Flag fusion proteins in transformed cells. DK168 cells were transformed with pRS316 (lane 1), pRS316-Flag-yNap1 (lane 2), pRS316-Flag-yNap1ΔNES (lane 3), or pRS316-Flag-yNap1 (L99A L102A) (lane 4). Exponentially growing transformants were lysed, and lysates were subjected to Western blot analysis with anti-Flag antibody. DK168 cells were separately transformed with pYES2 (lanes 5 and 6), pYES2-Flag-yNap1 (lanes 7 and 8), or pYES2-Flag-yNap1ΔNES (lanes 9 and 10). Lysates prepared from exponentially growing transformants in either SD (lanes 5, 7, and 9) or SG (lanes 6, 8, and 10) were subjected to Western blot analysis.