FIG. 5.

Complementation of a NAP1 disruptant with yNAP1 derivatives. The morphological change (A) and ts phenotype (B) of a NAP1-disrupted strain, DK168, were examined when DK168 was transformed with an empty vector [ORF(−)] (a) or expression vectors for Flag-yNap1 WT (WT) (b), Flag-yNap1ΔNES (ΔNES) (c), Flag-yNap1(L99A L102A) (L99A L102A) (d), Flag-yNap1ΔC1 (e), or Flag-yNap1ΔNESΔC1 (f). (A) The morphologies of exponentially growing transformants were observed under a phase microscope. (B) The ts phenotypes during growth were examined by incubation of serially diluted cell suspension spots at permissive (25°C) and nonpermissive (37°C) temperatures.