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Electrophoretic mobility-shift assay of HMG-D binding to normal and dU-containing oligonucleotides. The autoradiograms (A) were used to construct binding plots (B) in which v is the bound fraction of DNA. The x intercept yields an affinity constant of 87 nM for dT-containing oligonucleotides (•) and 19 nM for dU-containing oligonucleotides (○).
