FIG. 4.

Overexpressed p37 AUF1 is associated with AU-rich mRNAs in vivo. 293 cells were transfected with 1.0 μg of plasmids expressing β-Gal mRNAs and an ARE or GC reporter mRNA, 0.5 μg of a plasmid expressing Flag alone, or 0.5 or 2.0 μg of a plasmid expressing Flag-p37 AUF1. eIF4G and Flag were immunoprecipitated from cell lysates normalized to equal amounts of β-Gal mRNAs, and β-Gal mRNA-specific RT-PCR was carried out. The products of RT-PCR were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide.