FIG. 7.
Activation of GSTμ in 32Dcl3 cells following IL-3 withdrawal. (A) Northern blot analysis of total RNA extracted at the indicated times following IL-3 withdrawal from wild-type 32Dcl3 cells with a full-length GSTμ cDNA probe. As an internal control for RNA loading, blots were stained with ethidium bromide after completion of RNA transfer onto the nitrocellulose filter to compare levels of 18S and 28S rRNAs across lanes (shown below). (B) Comparison of GSTμ expression by Northern blotting in wild-type-, empty-vector-, p75c-myb-, and p89c-myb-transfected cells in the presence of IL-3 and at 9 h following IL-3 withdrawal. (C) Expression pattern of GSTμ in empty-vector-, p75c-myb-, p89c-myb-, and p75Δlz-c-myb-transfected cells. Total RNA was isolated from each cell line at the indicated time points following IL-3 withdrawal and subjected to Northern blotting.