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. 2003 Sep;23(18):6442–6454. doi: 10.1128/MCB.23.18.6442-6454.2003

FIG. 1.

FIG. 1.

NFAT is a target of p38 MAPK on the IL-2 gene promoter. (A) SB 203580 inhibited IL-2 production. Thymocytes and splenic T cells (5 × 105/well) from C57BL/6 mice were stimulated with ConA (2.5 μg/ml) plus anti-CD3 (10 μg/ml) or anti-CD28 (2.5 μg/ml) in the presence (SB) or absence (CTR [control]) of SB 203580 (10 μM). IL-2 produced was quantitated 24 h later with indicator cell line HT-2. (B) Transgenic p38α(AF) suppressed IL-2 production in T cells. Thymocytes and splenic T cells (5 × 105/well) from p38α(AF)-transgenic mice and NLC mice were stimulated with ConA (2.5 μg/ml) and CD3 plus CD28 at the concentrations indicated. IL-2 levels were determined 24 h after activation. (C) p38α(AF) inhibited the activation of NFAT-CAT in T cells. EL4 T cells were transfected with CAT reporters containing AP-1, NFAT, or NF-κB elements (18) and with or without 1 μg of p38α(AF) by the DEAE-dextran method. T cells were stimulated with TPA (10 ng/ml) plus A23187 (80 ng/ml) (T/A) 24 h later (or not stimulated [−]), and CAT activities were determined after another 8 h. Data are reported as means and standard errors of the means.