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. 2003 Sep;23(18):6442–6454. doi: 10.1128/MCB.23.18.6442-6454.2003

FIG. 5.

FIG. 5.

NFATc mRNA induction requires p38 MAPK. (A) NFATc mRNA induction in thymocytes was inhibited by SB 203580. Thymocytes were activated with TPA and A23187 (T/A) in the absence or presence of SB 203580 (10 μM) (SB). Thymocytes were harvested for RNA isolation 1, 2, and 4 h after activation. The amount of NFATc mRNA was determined by RT-PCR. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (B) Activation of the NFATc promoter was p38 MAPK dependent. A pGL2 reporter containing the NFATc promoter (−752 to −21 bp) was transfected into EL4 T cells without (CTR [control]) or with p38α(AF). T cells were activated with TPA and A23187 24 h later, and luciferase activity was determined 24 h after activation. −, no activation. RLU, relative light units. (C) MKK3b(E) alone induced NFATc expression in Jurkat cells. Jurkat T cells were transfected with active MKK3b(E) or pcDNA3 as a control. Cells transfected with pcDNA3 were then left untreated or were treated with TPA amd A23187 24 h later. The amount of NFATc mRNA was determined by RT-PCR.