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. 2003 Sep;23(18):6442–6454. doi: 10.1128/MCB.23.18.6442-6454.2003

FIG. 7.

FIG. 7.

Increased NFATc mRNA translation by p38 MAPK. 293T cells were collected 24 h after transfection with CMV-NFATc or CMV-NFATc plus MKK3b(E). Cell lysates were analyzed by sucrose gradient centrifugation. (A) Typical profile of the sucrose gradient monitored by measuring the absorbance at 254 nm. The top of the gradient, the 40S and 60S fractions, and polysome-containing fractions (1 to 10) are indicated. (B and C) Amounts of NFATc mRNA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in a polysome-containing fraction from 293T cells transfected with NFATc (B) or MKK3(E) plus NFATc (C) were determined by RT-PCR. (D) The distribution of the NFATc mRNA polysome-containing fraction was enhanced by p38 MAPK. NFATc mRNA in each polysome fraction was quantitated by real-time PCR. The quantity of NFATc mRNA was then plotted against the number of the polysome fraction.