Figure 3.

(A) Sequence of the human IL-15 5′ upstream region. The transcription initiation site and the NF-κB motif are in boldface type and underlined. Part of IL-15 exon 1, which is cloned in h15/sAP, is also underlined. (B) Schematic demonstration of some of the conserved motifs between mouse and human IL-15 promoters. (C) S1 nuclease protection assay to determine the transcription initiation site (+1 position). Total RNA from activated monocytes (by LPS and interferon γ) was used for the S1 nuclease assay, which was electrophoresed alongside the sequence ladder of the cloned IL-15 5′ upstream region to determine the +1 position.