Skip to main content
. 1998 Mar 3;95(5):2458–2462. doi: 10.1073/pnas.95.5.2458

Figure 1.

Figure 1

(A–C) Response of CD81-null mice to T dependent antigens varies with adjuvant. CD81-null mice (solid bars) and heterozygous littermates (hatched bars) were immunized i.p. with ovalbumin or KLH either precipitated in alum or given with QS-21 or IL-12 as adjuvants. Antigen-specific serum antibody responses were measured 12 weeks after a single immunization (primary) or 18 days after a second injection (boost). (A) Responses of CD81-null mice were significantly lower than heterozygotes to ovalbumin or KLH in alum, which induces a Th2 response. (B) Responses of CD81-null mice were not significantly different from heterozygotes when immunized with ovalbumin in QS-21 or KLH in IL-12, which stimulate a Th1 response. (C) The IgG1 response to ovalbumin in alum was significantly decreased in CD81-null mice. IgG2a responses to ovalbumin in alum were not significantly different between groups. (D) Response of CD81-null mice to a T independent antigen. CD81-null mice (▪) and heterozygous littermates (□) were immunized i.p. with 25 μg of TNP-Ficoll. The serum antibody response to TNP was measured by ELISA at 7 and 14 days. There were no significant differences between groups. Data are the means ± SEM (n = 3 to 9 animals per group).