Figure 3.

Antigen-specific IL-4 and IFN-γ responses in CD81-null mice (solid bars) and heterozygous littermates (hatched bars). Mice were immunized three times with the specified antigen in alum. Spleens and peripheral lymph nodes were removed 4 days after the last injection. Mononuclear cells were restimulated with ovalbumin or KLH for 3 days, then supernatants were harvested, and cytokines measured by ELISA (25). Bars indicate the SD of triplicate cultures.