TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Bacteria | ||
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 21 |
| JM101 | F′ traD36 proA+B+laclq Δ(lacZ)M15/Δ(lax-proAB) glnV thi | New England Biolabs |
| DH5α/pRK212.1 | contains conjugative plasmid for conjugation of plasmid DNA into Campylobacter | 14 |
| C. jejuni | ||
| 11168 | clinical isolate used for genome sequencing | 43 |
| 81-176 | clinical isolate | 32 |
| DRH153 | 81-176 astA::aphA3; Knr | 22 |
| DRH154 | 81-176 astA::cat; Cmr | This study |
| DRH212 | 81-176 rpsLSm | This study |
| DRH312 | 11168 gyrANA | This study |
| DRH335 | 81-176 gyrANA | This study |
| RSW42 | DRH212 ctsE::cat-rpsL | This study |
| RSW44 | DRH212 ctsX::cat-rpsL | This study |
| RSW46 | RSW44 ΔctsX, in-frame deletion of codons 5 to 190 of CtsX | This study |
| RSW81 | DRH212 ctsP::cat-rpsL | This study |
| RSW82 | DHR212 ctsR::cat-rpsL | This study |
| RSW115 | RSW81 ΔctsP, in-frame deletion of codons 6 to 196 of CtsP | This study |
| RSW136 | RSW42 ΔctsE, in-frame deletion of codons 7 to 514 of CtsE | This study |
| RSW137 | RSW82 ΔctsR, in-frame deletion of codons 4 to 93 of CtsR | This study |
| Plasmids | ||
| pUC19 | Ampr | New England Biolabs |
| pDRH138 | pUC19 with 2.3-kb fragment containing astA cloned into PstI site | This study |
| pDRH142 | pDRH138 with kanamycin cassette cloned into EcoRV site of astA | This study |
| pDRH143 | pDRH138 with chloramphenicol cassette cloned into EcoRV site of astA | This study |
| pDRH265 | pUC19 with 1.4 kb cat-rpsL cloned into SmaI site | 22 |
| pDRH328 | pUC19 with coding sequence of gyrA NAr in BamHI site | This study |
| pRY108 | Campylobacter-E. coli shuttle vector; Knr | 66 |
| pRY109 | pUC19 with Campylobacter cat cassette | 66 |
| pRY112 | Campylobacter-E. coli shuttle vector; Cmr | 66 |
| pILL600 | contains Campylobacter kanamycin cassette | 33 |
| pECO102 | pRY112 derivative with cat promoter in XhoI-BamHI site | This study |
| pRSW100 | pUC19 with 2.8-kb fragment harboring ctsE cloned into XmaI site | This study |
| pRSW101 | pUC19 with 2-kb fragment harboring ctsX cloned into XmaI site | This study |
| pRSW102 | pRSW100 with cat-rpsL cloned into NruI site of ctsE | This study |
| pRSW103 | pRSW101 with cat-rpsL cloned into BsgI site of ctsX | This study |
| pRSW105 | pUC19 with ctsE in-frame deletion construct cloned into XmaI site | This study |
| pRSW106 | pUC19 with ctsX in-frame deletion construct cloned into XmaI site | This study |
| pRSW107 | pRSW104 with cat-rpsL cloned into EcoRV site of cj0011c | This study |
| pRSW108 | pUC19 with 1.6-kb fragment with ctsR cloned into XmaI site | This study |
| pRSW110 | pUC19 with 1.6-kb fragment with ctsP cloned into XmaI site | This study |
| pRSW112 | pRSW108 with cat-rpsL cloned into XmaI site of ctsR | This study |
| pRSW113 | pRSW110 with cat-rpsL cloned into XmaI site of ctsP | This study |
| pRSW114 | pUC19 with ctsP in-frame deletion construct cloned into XmaI site | This study |
| pRSW115 | pUC19 with ctsR in-frame deletion construct cloned into XmaI site | This study |
| pRSW119 | pECO102 with ctsF coding sequence (codon 2-stop) cloned into BamHI/XhoI site | This study |
| pRSW120 | pECO102 with ctsE coding sequence (codon 2-stop) cloned into BamHI/XhoI site | This study |
| pRSW121 | pECO102 with ctsX coding sequence (codon 2-stop) cloned into BamHI/XhoI site | This study |
| pRSW122 | pECO102 with ctsP coding sequence (codon 2-stop) cloned into BamHI/XhoI site | This study |