TABLE 2.
Location of solo in C. jejuni transformation mutants
Transposon locationa | Coding sequence descriptionb | Transformation efficiencyc |
---|---|---|
NAd-81-176 | NA | 2.3 × 10−3 |
cj1352 (ceuB) | Enterochelin uptake permease | 6.70 × 10−8 |
cj1343c (ctsG) | Putative periplasmic protein | 8.00 × 10−8 |
cj1076 (proC) | Putative PCA reductase | 2.5 × 10−7 |
cj1077 (ctsT) | Putative periplasmic protein | 3.32 × 10−7 |
3′ of ansA (C23) | Not in a coding sequence | 5.47 × 10−8 |
cj1028c (ctsW) | Purine/pyrimidine phosphoribosyltransferase | <10−9 |
cj1473c (ctsP) | Putative ATP/GTP binding protein | 2.48 × 10−7 |
cj1475c (ctsR) (2) | Hypothetical protein | 1.78 × 10−7-1.60 × 10−8 |
cj1471c (ctsE) (4) | Putative type II secretion system E protein | 1.51 × 10−7-6.00 × 10−7 |
cj1470c (ctsF) | Pseudogene type II secretion system F protein | 2.94 × 10−7 |
cj1474c (ctsD) (4) | Putative type II secretion system D protein | 1.37 × 10−7-9.04 × 10−8 |
The number in parentheses indicates the number of independent solo insertions identified in each gene
The gene designation and proposed function are based on the annotated genome sequence of C. jejuni strain 11168 (43).
The transformation efficiency is calculated as the number of transformants per total number of CFU per microgram of DNA.
NA, not applicable.