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Purification of ISPs
| ISP | Protein yield (mg) | Sp act (U/mg)a | Fe content (Fe/α3β3) | S content (S/α3β3) |
A280/A323
|
|
|---|---|---|---|---|---|---|
| Oxidized | Reduced | |||||
| αBβB | 75 | 5.0 | 11 | 6.0 | 6.1 | 12.7 |
| αTβT | 39 | 3.8 | 16 | 5.8 | 4.5 | 6.7 |
| αTβB | 100 | 2.3 | 15 | 5.9 | 4.5 | 6.7 |
| αBβT | 32 | 3.1 | 12 | 5.9 | 6.1 | 12.7 |
Activity units are defined in Materials and Methods. Specific activities were obtained with 3-methylbenzoate for αTβT and αTβB and with benzoate for αBβB and αBβT.
