TABLE 6.

Coupling of substrate utilization in TADO_mt2, BADO_ADP1, and their hybrids^a

Enzyme	Substrate	Substrate/O2 ratio	H2O2/O2 ratio
αBβB	Benzoate	1.02 (0.08)	0.01 (0.002)
	2-Methylbenzoate	1.01 (0.10)	0.04 (0.01)
	3-Methylbenzoate	1.04 (0.12)	0.02 (0.002)
	3-Chlorobenzoate	1.04 (0.10)	0.002 (0.0008)
αTβTb	Benzoate	0.96 (0.10)	0.03 (0.006)
	2-Methylbenzoate	0.10 (0.10)	0.27 (0.02)
	3-Methylbenzoate	0.92 (0.13)	0.01 (0.003)
	4-Methylbenzoate	0.90 (0.10)	0.03 (0.005)
	2-Chlorobenzoate	0.10 (0.09)	0.2 (0.05)
	3-Chlorobenzoate	0.94 (0.10)	0.002 (0.0005)
	4-Chlorobenzoate	0.93 (0.10)	0.03 (0.01)
αTβB	Benzoate	0.98 (0.10)	0.02 (0.003)
	2-Methylbenzoate	0.92 (0.10)	0.04 (0.002)
	3-Methylbenzoate	0.98 (0.13)	0.007 (0.001)
	4-Methylbenzoate	0.94 (0.10)	0.01 (0.003)
	2-Chlorobenzoate	0.90 (0.09)	0.05 (0.01)
	3-Chlorobenzoate	0.96 (0.10)	0.03 (0.0006)
	4-Chlorobenzoate	0.97 (0.10)	0.04 (0.007)
αBβT	Benzoate	1.02 (0.10)	0.03 (0.003)
	2-Methylbenzoate	1.01 (0.10)	0.02 (0.02)
	3-Methylbenzoate	1.04 (0.13)	0.01 (0.002)
	3-Chlorobenzoate	1.01 (0.10)	0.02 (0.0003)

^aExperiments were performed with air-saturated 100 mM sodium phosphate (pH 7.0 at 25°C) containing 430 μM NADH, 4 μM RED, 1.8 μM ISP, and 215 μM substrate. Standard errors are given in parentheses. The reductase in the assay was paired to the α subunit of the ISP.

^bValues published in reference 20.