FIG. 1.

Intercellular complementation of Tafi between S. enterica serovar Enteritidis 3b ΔagfB donor and ΔagfA recipient strains. CR binding of different ΔagfA recipient strains grown together with ΔagfB donor strains is shown. Recipient strain genotypes are indicated above each graph, while ΔagfB donor strain phenotypes are listed at the bottom. CR binding values for each combination of mutants were normalized by subtracting values for the corresponding ΔagfB or ΔagfA strains grown individually. Each bar shows the averages and standard errors from four separate experiments, and asterisks indicate significant differences within each group (*, P < 0.05; **, P < 0.01; ***, P < 0.001 [Tukey-Kramer multiple comparisons test]). The panels below the bar graphs show immunoblot analysis performed with immune serum raised to purified Tafi. The amount of AgfA fimbrial material associated with recipient and donor cells scraped off the TCR agar is noted for each recipient-donor combination.