TABLE 1.

Morphological characterization of S. enterica serovar enteritidis 3b and isogenic agfA or agfB mutants

Straina	Colony colorb	Morphologyc	S. enterica serovar Typhimurium morphologyd	CR bound (μg)e
3b (agfBA+)	Red	Ag(++)	rdar	35.8 ± 9.9
ΔbcsA	Red-brown	Ag(++)	bdar	44.1 ± 12.3
galE::Tn10	Orange-brown	Ag(+)	N/Af	34.7 ± 6.1
ΔbcsA galE::Tn10	Orange-brown	Ag(+)	N/A	34.5 ± 2.9
ΔagfA mutant	Pink	NAg	pdar	5.6 ± 0.6
ΔbcsA	White	NAg	saw	3.7 ± 0.4
galE::Tn10	Pink-orange	NAg	N/A	8.4 ± 1.5
ΔbcsA galE::Tn10	Pink	NAg	N/A	7.3 ± 1.5
ΔagfB mutant	Pink	NAg	pdar	4.6 ± 0.6
ΔbcsA	White	NAg	saw	3.6 ± 0.6
galE::Tn10	Pink-orange	NAg	N/A	11.2 ± 2.2
ΔbcsA galE::Tn10	Pink	NAg	N/A	10.6 ± 1.1

^aS. enteritidis strains as described in Materials and Methods.

^bColor of colonies judged after growth on TCR agar for 24 h at 37°C.

^cThe morphology of colonies was compared to that of the 3b parent strain Ag(++), most aggregative (colonies dry and difficult to break apart); Ag(+), aggregative (colonies dry but easier to break apart); NAg, nonaggregative (colonies mucoid).

^dColony phenotypes of analogous S. enterica serovar Typhimurium strains grown on Luria agar without salt (containing 40 μg of CR per ml and 20 μg of Coomassie brilliant blue per ml) rdar, red, dry, and rough; bdar, brown, dry, and rough; pdar, pink, dry, and rough; saw, smooth and white. Phenotypes are as reported previously (35, 36, 48).

^eAmount of CR eluted from 10 A₆₀₀ units of cells ± standard error of the mean from four independent experiments.

^fN/A, not applicable.