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Plasmid pE3R carrying the pmsCEAB operon of P. fluorescens. Plasmid pE3R was cut with different restriction enzymes and religated. E, EcoRI; A, ApaI; S, SacII; St, StuI; St*, methylated StuI cutting site. Arrows indicate genes and the direction of transcription. Dashed lines denote generated deletions. The resulting plasmids were designated pPmsCB (cut with ApaI/SacII, harboring pmsCB), pPmsC (cut with ApaI/NdeI, harboring pmsC), and pPmsB (cut with StuI/SacII, harboring pmsB).
