TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype and/or phenotype | Reference or source |
|---|---|---|
| Y. enterocolitica | ||
| WA-C | Derivative of Y. enterocolitica, BG1B, serotype O:8, WA-314, pYV plasmid cured; Nalr | 12 |
| WA-CS | Sm resistant derivative of WA-C; Nalr Smr | 17 |
| WA-CSirp1::Kanr | irp1 mutant of WA-CS, Ybt deficient strain; Smr Kanr Nalr | 17 |
| WA-CSirp9::Kanr | WA-CS carrying a kanamycin cassette without transcriptional terminator in gene irp9; Smr Kanr Nalr | This study |
| WA-CSG | WA-CS carrying reporter construct pCJFY5G3; Nalr Cmr | This study |
| WA-CSirp1::KanrG | WA-CSirp1::Kanr carrying reporter construct pCJFY5G3; Smr Kanr Nalr Cmr | This study |
| WA-CSirp9::KanrG | WA-CSirp9::Kanr carrying reporter construct pCJFY5G3; Smr Kanr Nalr Cmr | This study |
| E. coli | ||
| S17-1 λpir | pir+tra+ | 14, 23 |
| PBB7 | entC mutant, enterobactin deficient, CAS-negative phenotype | 15 |
| BL21(DE3) | Enterobactin positive carrying IPTG-inducible T7 polymerase gene | Stratagene |
| Plasmids | ||
| pSB315 | Containing kanamycin cassette without transcriptional terminator; Ampr Kanr | 10 |
| 12H2 | pLAFR2 carrying a DNA insert of WA-C comprising irp6-9 operon, ybtA, and irp2; Tcr | 2, 19 |
| pKAS32 | Suicide vector with rpsL gene; Ampr | 24 |
| pSVIrp9 | pKAS32 carrying a SalI/HpaI DNA-fragment of the HPI with, a kanamycin cassette (without transcriptional terminator) integrated into the SphI site of irp9; Ampr Kanr | This study |
| pGP1-2 | Vector containing heat-inducible T7 polymerase; Kanr | 25 |
| pT7-5 | Cloning vector; Ampr | 25 |
| pTlrp9 | pT7-5 vector carrying irp9 (SalI/PstI fragment of cosmid 12H2) upstream of T7 promoter; Ampr | This study |
| pCJFY5G3 | Reporter construct, pACYC184 carrying 153 bp of fyuA promoter region fused to gfp:Cmr | 2 |
| pE3R | pGEM-3Z carrying the pmsCEAB operon of P. fluorescens WCS374; Ampr | 13 |
| pE3 | pGEM-3Z carrying the T7 promoter upstream of the pmsCEAB operon of P. fluorescens WCS374; Ampr | 13 |
| pPmsCB | pGEM-3Z carrying pmsC and pmsB genes (ApaI/SacII fragment of pE3R) of P. fluorescens WCS374; Ampr | This study |
| pPmsC | pGEM-3Z carrying pmsC gene (ApaI/NdeI fragment of pE3R) of P. fluorescens WCS374; Ampr | This study |
| pPmsB | pGEM-3Z carrying pmsB gene (StuI/SacII fragment of pE3R) of P. fluorescens WCS374; Ampr | This study |
| pUC57 | Cloning vector; Ampr | MBI Fermentas |
| pUCEntC | pUC57 carrying entC of E. coli; Ampr | This study |