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. 2003 Sep;185(18):5483–5490. doi: 10.1128/JB.185.18.5483-5490.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 2. — LC/MS analysis of nucleosides from unfractionated tRNA of S. hydrogenophila cultured at 90°C. Components: 1, Ψ; 2, m⁵C; 3, m¹A; 4, Cm; 5, I; 6, m⁷G; 7, o⁸G (non-tRNA nucleoside; see the text); 8, Um; 9, m⁵Cm; 10, m¹I; 11, m¹G; 12, Gm; 13, ac⁴C; 14, m²G; 15, t⁶A; 16, m₃G isomer of unknown structure; 17, G; 18, Am; 19, m24Cm (non-tRNA nucleoside, see the text); 20, ac⁴Cm; 21, m²A; 22, m⁶A; 23, m²Gm; 24, hn⁶A; 25, ms²t⁶A; 26, m^2,7Gm; 27, imG; 28, Gm; 29, hn⁶A + CH₃; ms²hn⁶A; 30, 31, imG isomer of unknown structure; 32, unknown nucleoside N415; 33, A (non-tRNA nucleoside, see text); 34, mimG.

Inline graphic — LC/MS analysis of nucleosides from unfractionated tRNA of S. hydrogenophila cultured at 90°C. Components: 1, Ψ; 2, m⁵C; 3, m¹A; 4, Cm; 5, I; 6, m⁷G; 7, o⁸G (non-tRNA nucleoside; see the text); 8, Um; 9, m⁵Cm; 10, m¹I; 11, m¹G; 12, Gm; 13, ac⁴C; 14, m²G; 15, t⁶A; 16, m₃G isomer of unknown structure; 17, G; 18, Am; 19, m24Cm (non-tRNA nucleoside, see the text); 20, ac⁴Cm; 21, m²A; 22, m⁶A; 23, m²Gm; 24, hn⁶A; 25, ms²t⁶A; 26, m^2,7Gm; 27, imG; 28, Gm; 29, hn⁶A + CH₃; ms²hn⁶A; 30, 31, imG isomer of unknown structure; 32, unknown nucleoside N415; 33, A (non-tRNA nucleoside, see text); 34, mimG.