Figure 1. Characterization of mouse models.

Representative Western blots showing (A) levels of exogenous human Bcl-2 (hBcl-2), endogenous mouse Bcl-2 (mBcl-2), and total (human and mouse) Bcl-2 (ttBcl-2) as well as (B) levels of mouse TNF in cytosolic and mitochondrial protein extracts of 12-week-old littermate control (LM), MHCsTNF, MHCsTNF/Bcl-2, and Bcl-2 mouse hearts. GAPDH (cytosol) and COX IV (mitochondria) served as loading normalization. (C) Myocardial TNF protein levels were measured by ELISA. **P < 0.05 compared with LM.