Figure 4. Myocardial cytochrome c release.

The release of cytochrome c from (A) mitochondria to (B) the cytosol was determined by Western blotting in 12-week-old littermate control, MHCsTNF, MHCsTNF/Bcl-2, and Bcl-2 mouse hearts. Shown are representative Western blots next to the group data for mitochondrial cytochrome c levels (normalized to COX IV) and cytosolic cytochrome c levels (normalized to GAPDH). Group data represent the ratio of experimental (transgenic) to control (wild-type) mouse groups. *P < 0.05 between MHCsTNF and MHCsTNF/Bcl-2 groups; **P < 0.05 compared with LM.