Figure 5. Myocardial levels of proapoptotic proteins.

(A) The release of mitochondrial Smac/Diablo, Omi/HtrA2, apoptosis-inducing factor (AIF), and Endo G into the cytosol was determined by Western blotting in littermate control, MHCsTNF, MHCsTNF/Bcl-2, and Bcl-2 mouse hearts at 12 weeks of age. (B) Group data for the corresponding protein levels (normalized to GAPDH) are expressed as the ratio of experimental (transgenic) to control (wild-type) mouse groups. *P < 0.05 between MHCsTNF and MHCsTNF/Bcl-2 groups; **P < 0.05 compared with LM.