Figure 7. Myocardial caspase-8 activation, Bid, and IAPs.

(A) Caspase-8–like activity was determined in 12-week-old littermate control, MHCsTNF, MHCsTNF/Bcl-2, and Bcl-2 mouse hearts using a fluorogenic assay. Cytosolic levels of (B) full-length Bid (23 kDa) and t-Bid (15 kDa) and of (C) c–IAP-1 and c–IAP-2 as determined by Western blotting. Group data for the corresponding levels (normalized to GAPDH) are shown as the ratio of experimental (transgenic) to control (wild-type) mouse groups. x, empty lane. *P < 0.05 compared with LM.